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Immunologie et infection

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通过转版免疫沉淀和质谱测定,在HIV-1复制过程中核子因子的识别

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Immunologie et infection

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Journal JoVE Immunologie et infection

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

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通过转版免疫沉淀和质谱测定,在HIV-1复制过程中核子因子的识别

DOI:

10.3791/59329-v

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09:38 min

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June 26, 2019

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Jerlisa Ann C. Arizala², Pritsana Chomchan, Haitang Li, Roger Moore, Helen Ge, Dominique L. Ouellet, John J. Rossi²

¹Molecular and Cellular Biology Department,Beckman Research Institute at the City of Hope, ²Irell & Manella Graduate School of Biological Sciences, ³Department of Molecular Immunology,Beckman Research Institute and the City of Hope

Chapitres

00:04Titre
00:47Rev-nucleolar Localization Signal (NoLS)-3’Flag Mutation Expression During HIV-1 Replication
02:03Viral Protein Lysate Collection
03:12Rev-NoLS-3’Flag Coimmunoprecipitation
05:20In-gel Reduction, Alkylation, and Digestion of Coomassie-stained Gel Bands
07:14Results: Representative Nucleolar Factor Identification During HIV-1 Replication
09:05Conclusion

Summary

Traduction automatique

在这里,我们描述了在质谱中存在HIV-1复制的Rev免疫沉淀。所述方法可用于识别艾滋病毒-1感染周期中涉及的核素因子,并适用于其他疾病模型,用于对研究不足的途径进行表征。

Tags

Keywords: HIV-1 Nucleolar Factors Rev Immunoprecipitation Mass Spectrometry Viral Factors Host Factors Infectious Cycle Protein Characterization HeLa Cells Plasmid Transfection Cell Lysis Protease Inhibitor Immunoprecipitation

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