/

/

冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Please note that all translations are automatically generated. Click here for the English version.

冷冻斑马鱼胚胎的连续免疫荧光与免疫组化学

DOI:

10.3791/59344-v

•

09:20 min

•

May 14, 2019

•

Jordan L. Ferguson*, Heather R. Shive*

¹Department of Population Health and Pathobiology,NC State University College of Veterinary Medicine

Chapitres

00:04Titre
01:06Embryo Embedding and Preparation of Cryosections
02:31Immunofluorescence for pH3
04:54Immunohistochemistry for Labeled Dextran
07:50Results: Identification of Individual Cells After Immunofluorescence and Immunohistochemistry
08:21Conclusion

Summary

Traduction automatique

该方案演示了早期斑马鱼胚胎的冷冻节的连续免疫荧光和免疫组织化学,从而对特定细胞群进行精确的共定位分析。

Tags

Immunofluorescence Immunohistochemistry Cryosections Zebrafish Embryos Co-localization Antibody Compatibility Cryosectioning Co-expression Embryo Orientation Cryostat Charged Glass Slides PBS Wash Humid Chamber Barrier Pen

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

使用荧光抗体对小鼠表皮石蜡切片的免疫组织化学

显微注射mRNA和斑马鱼的胚胎吗啉反义寡核苷酸。

斑马鱼的胚胎显微注射来分析基因的功能

双光子现场斑马鱼的胚胎干切断和时间推移共焦成像

移植嵌合斑马鱼胚胎生成

在现场的斑马鱼胚胎的基于双光子Photoactivation

腹腔注射到成年斑马鱼

在斑马鱼胚胎的代谢标记和Bioorthogonal点击化学成像聚糖

免电力，顺序的核酸和蛋白质的分离

斑马鱼胚胎的代谢谱分析

Read Article