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构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
DOI:

08:13 min

April 08, 2019

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Chapitres

  • 00:04Titre
  • 00:28Preliminary Setup
  • 01:11Setting up the Detection Path
  • 02:14Setting up the Excitation Path and the Cylindrical Lenses
  • 04:59Testing Tile Imaging
  • 05:53Highly Inclined Swept Tile (HIST) Imaging
  • 06:52Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
  • 07:38Conclusion

Summary

Traduction automatique

详细介绍了如何建立高倾斜的扫描瓷砖 (HIST) 显微镜及其在单分子成像中的应用。

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