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枯草芽孢杆菌中细菌体和内膜的可视化
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
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枯草芽孢杆菌中细菌体和内膜的可视化

DOI:
10.3791/59388-v

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08:58 min

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April 15, 2019

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, , , ,
1Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences,University of Amsterdam, 2Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences,University of Amsterdam, 3Confocal.nl BV., 4Dept. of Molecular Biology and Biophysics,UConn Health

Chapitres

  • 00:04Titre
  • 00:34High Precision Coverslip and Glass Slide Preparation
  • 01:17Fluorescent Microsphere and Spore Sample Mounting
  • 02:32Fluorescent Microsphere and Spore Imaging
  • 03:38FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
  • 05:00Image Analysis
  • 06:25Results: Representative Germinosome and Inner Membrane Visualization
  • 08:04Conclusion

Summary

Traduction automatique

Traduction automatique

萌发细胞受体蛋白聚集在枯草芽孢杆菌内膜的 "生殖细胞" 中。我们描述了一个协议, 使用超级分辨率显微镜和荧光报告蛋白可视化生殖细胞。该协议还确定了优先与 fm4-64 的膜染料染色的孢子内膜域。

Tags

GerminosomesInner MembraneBacillus Subtilis SporesSuper-resolution Structured Illumination Microscopy3D-SIMFluorescent Reporter ProteinsSpore MembranesLipid DomainsCoverslipsMicroscope SlidesFluorescent MicrospheresAgaroseUltrapure WaterGene FrameStructured Illumination MicroscopePoint Spread FunctionExcitation Wavelengths

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