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Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays
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Journal JoVE Biochimie
Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

DOI:
10.3791/59390-v

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11:17 min

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June 13, 2019

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, ,
1Oryzon Genomics

Chapitres

  • 00:04Titre
  • 01:05Solution Preparation
  • 02:11Native Protein Extraction
  • 03:51Luminescent ELISAs for Total and Free KDM1A Determination
  • 08:42Results: Direct Measurement of KDM1A Target Engagement
  • 10:32Conclusion

Summary

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Traduction automatique

Here, we present a protocol to measure KDM1A target engagement in a human or animal cell, tissue or blood samples treated with KDM1A inhibitors. The protocol employs chemoprobe tagging of the free KDM1A enzyme and direct quantification of the target occupation using chemoprobe-based immunoassays and can be used in preclinical and clinical studies.

Tags

Target EngagementKDM1AChemoprobe-based ImmunoassayIadademstatORY-1001PharmacodynamicsDrug DevelopmentCell LysisProtease Inhibitor

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