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Immunologie et infection
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Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test

Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test

DOI:
10.3791/59416-v

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08:58 min

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April 30, 2019

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, , , , ,
1Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Knoxville, TN,United States Department of Agriculture (USDA), 2Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Sutton, MA,United States Department of Agriculture (USDA), 3Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Milton, FL,United States Department of Agriculture (USDA), 4Animal and Plant Health Inspection Service (APHIS), Wildlife Services, Concord, NH,United States Department of Agriculture (USDA), 5Lyssa LLC

Chapitres

  • 00:04Titre
  • 00:41Brainstem Collection
  • 01:41Preparation of Materials for DRIT
  • 03:10Direct Rapid Immunohistochemical Test
  • 06:43Results: Analysis of the Direct Rapid Immunohistochemical Test
  • 08:20Conclusion

Summary

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The direct rapid immunohistochemical test (DRIT) offers a World Organization for Animal Health and World Health Organization (OIE/WHO) recognized alternative to the direct fluorescent antibody (DFA) test for rabies diagnosis. This test allows for field-based applications that can be performed in approximately 1 h upon brain impressions using light microscopy.

Tags

Keywords: RabiesDRITDirect Rapid Immunohistochemical TestRabies DiagnosisRabies SurveillanceRabies PreventionRabies ControlLight MicroscopyDecentralized LaboratoriesField BiologistsBrainstem SamplesPhosphate Buffered FormalinHydrogen PeroxideHematoxylinAmino-ethyl-carbazoleAcetate Buffer

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