Journal
/
Biologie du développement
/
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors

DOI:
10.3791/59432-v

•

07:44 min

•

September 14, 2019

•

, , , ,
1Medical College of Georgia,Augusta University

Chapitres

  • 00:04Titre
  • 01:45Selecting and Harvesting ES-like Cells
  • 03:02Deletion of Exon 23 in Mouse iPSCs with Two Guide RNAs (gRNAs) coupled with Cas9
  • 05:25Identification of iPSC Colonies with Exon23 Deletion
  • 06:33Results: CRISPR/Cas9-mediated Exon23 Deletion
  • 06:57Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a Cas9-based exon23 deletion protocol to restore dystrophin expression in iPSC from Dmdmdx mouse-derived skin fibroblasts and directly differentiate iPSCs into myogenic progenitor cells (MPC) using the Tet-on MyoD activation system.

Tags

CRISPR/Cas9DystrophinDuchenne Muscular DystrophyInduced Pluripotent Stem CellsMuscle Progenitor CellsGene EditingStem Cell TherapyAutologous CellsCongenital Diseases

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59432?article_type=v

Ex Vivo Culture of Pharyngeal Arches to Study Heart and Muscle Progenitors and Their Niche
check_url/fr/59432?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/fr/59432?article_type=v

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
check_url/fr/59432?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/fr/59432?article_type=v

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders
check_url/fr/59432?article_type=v

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
check_url/fr/59432?article_type=v

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
check_url/fr/59432?article_type=v

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
check_url/fr/59432?article_type=v

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
check_url/fr/59432?article_type=v

An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors

Read Article