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An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
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Journal JoVE Neurosciences
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

DOI:
10.3791/59433-v

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05:51 min

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May 23, 2019

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, , ,
1Department of Neurosciences,University of California, San Diego

Chapitres

  • 00:04Titre
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

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This protocol details a procedure in which human neuronal cultures are transduced with lentiviral constructs coding for mutant human tau. Transduced cultures display tau aggregates and associated pathologies.

Tags

Keywords: Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

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