Journal
/
Biochimie
/
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
Journal JoVE
Biochimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biochimie
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity

DOI:
10.3791/59452-v

•

08:00 min

•

July 10, 2019

•

, , , , , , , , , ,
1Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering,University of Alabama at Birmingham, 3Department of Cardiac Surgery,The Second Xiangya Hospital of Central South University

Chapitres

  • 00:04Titre
  • 01:13hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
  • 03:42Calcium Transient and Contractility Recording
  • 05:21Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
  • 07:11Conclusion

Summary

Traduction automatique

Traduction automatique

In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.

Tags

Keywords: HiPSC-derived CardiomyocytesRho Kinase ActivityCell EngraftmentCell AdhesionCell SurvivalCardiac InfarctionHeart FailureY-27632VerapamilCell TransplantationCell DissociationCell CultureCardiac FunctionVascularizationApoptosis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59452?article_type=v

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
check_url/fr/59452?article_type=v

Assaying Protein Kinase Activity with Radiolabeled ATP
check_url/fr/59452?article_type=v

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
check_url/fr/59452?article_type=v

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
check_url/fr/59452?article_type=v

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
check_url/fr/59452?article_type=v

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
check_url/fr/59452?article_type=v

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
check_url/fr/59452?article_type=v

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
check_url/fr/59452?article_type=v

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
check_url/fr/59452?article_type=v

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Read Article