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Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue
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Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

DOI:
10.3791/59471-v

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07:09 min

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May 09, 2019

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, , , , ,
1Department of Biological and Environmental Sciences,Longwood University, 2Department of Chemistry and Physics,Longwood University

Chapitres

  • 00:04Titre
  • 00:47Restriction Enzyme Digestion
  • 01:50Ligation
  • 02:29Colony PCR
  • 03:14Titrating E. coli to Check Function of Device
  • 03:54Using E. coli as Chemical Sensor for GSR
  • 04:44Fluorescence Analysis Using Portable Spectrometer
  • 05:09Fluorescence Analysis Using 96-well Plate Reader
  • 05:34Results: Analysis of the Biosensor and Presumptive Detection of Gunshot Residue
  • 06:29Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

Tags

Keywords: Bacterial BiosensorGunshot ResidueForensic ApplicationsSynthetic BiologyPlasmid DNARestriction EnzymesDNA LigationBacterial TransformationPCR

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