Journal
/
Immunologie et infection
/
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

DOI:
10.3791/59478-v

•

10:59 min

•

May 12, 2019

•

, , , , ,
1Wellcome Trust Sanger Institute, 2Department of Medicine,University of Cambridge, 3University of Cardiff

Chapitres

  • 00:04Titre
  • 00:38Differentiation from iPSCs to the Hindgut
  • 01:10Embedding of the Hindgut in Basement Membrane Matrix
  • 03:13Maintenance, Passage, and Pre-stimulation of iHOs with rhIL-22
  • 05:28Microinjection of iHOs and Intracellular Invasion Assays
  • 09:19Results: Studying Epithelial Cell Protection Against Pathogens Using hiPSC-derived iHOs
  • 10:05Conclusion

Summary

Traduction automatique

Traduction automatique

Human induced pluripotent stem cell (hiPSC)-derived intestinal organoids offer exciting opportunities to model enteric diseases in vitro. We demonstrate the differentiation of hiPSCs into intestinal organoids (iHOs), the stimulation of these iHOs with cytokines, and the microinjection of Salmonella Typhimurium into the iHO lumen, enabling the study of an epithelial invasion by this pathogen.

Tags

Keywords: Human Induced Pluripotent Stem CellsIntestinal OrganoidsEpithelial Cell ProtectionSalmonellaPathogensMicro-injectionDifferentiationRPMI-B27 MediaCHIR99021Retinoic AcidBasement Membrane MatrixCollagenaseOrganoid Base Growth MediaCentrifugation24-well Plate

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59478?article_type=v

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
check_url/fr/59478?article_type=v

Clinical Application of Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood
check_url/fr/59478?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/fr/59478?article_type=v

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
check_url/fr/59478?article_type=v

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
check_url/fr/59478?article_type=v

Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
check_url/fr/59478?article_type=v

Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
check_url/fr/59478?article_type=v

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
check_url/fr/59478?article_type=v

Opsonophagocytic Killing Assay to Assess Immunological Responses Against Bacterial Pathogens
check_url/fr/59478?article_type=v

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Read Article