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비 환 원 SDS-PAGE 분석 및 화학적 가교 결합으로 포유류 세포에서 이황화 물 조합에 의해 안정화 된 다이 성체 복합체를 배양에 검출
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Chapitres
- 00:04Titre
- 00:34Harvesting Cells After Blocking Free Cysteine Residues Using Iodoacetamide
- 02:02Extraction of Protein and Sample Preparation
- 03:13In Vivo Formaldehyde Cross-linking of Endogenous Proteins in U-2 OS Cells
- 04:30Fractionation of Nuclei
- 06:11Extraction of Protein and Sample Preparation
- 06:51SDS-PAGE Analysis
- 07:53Results: Analyzing Multimeric Complexes Using a Two-step Method of Non-reducing SDS-PAGE Analysis and Formaldehyde Cross-linking
- 09:04Conclusion
