Journal
/
Recherche en cancérologie
/
Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

DOI:
10.3791/59502-v

•

06:29 min

•

June 07, 2019

•

, ,
1Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, 2Department of Medicine,Harvard Medical School

Chapitres

  • 00:04Titre
  • 00:34Preoperative Preparation
  • 01:48Intraductal Injection
  • 03:53Results: Key Features of Genetic Manipulations of Mammary Epithelial Cells Based on Intraductal Injection of Ad-Cre
  • 05:25Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of this protocol is to describe a new breast cancer modeling approach based on the intraductal injection of Cre-expressing adenovirus into mouse mammary glands. This approach allows both cell-type- and organ-specific manipulation of oncogenic events in a temporally controlled manner.

Tags

Keywords: Breast CancerMouse ModelIntraductal InjectionCre-expressing AdenovirusMammary GlandMammary Epithelial CellsSurgical ProcedureAnesthesiaAnalgesiaHair RemovalDisinfectionIncisionNipple RemovalHamilton SyringeDyeDuctal Tree

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59502?article_type=v

Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis
check_url/fr/59502?article_type=v

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
check_url/fr/59502?article_type=v

Intracarotid Cancer Cell Injection to Produce Mouse Models of Brain Metastasis
check_url/fr/59502?article_type=v

Sectioning Mammary Gland Whole Mounts for Lesion Identification
check_url/fr/59502?article_type=v

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
check_url/fr/59502?article_type=v

Orthotopic Injection of Breast Cancer Cells into the Mice Mammary Fat Pad
check_url/fr/59502?article_type=v

Rat Mammary Epithelial Cell Transplantation into the Interscapular White Fat Pad
check_url/fr/59502?article_type=v

Modeling Brain Metastases Through Intracranial Injection and Magnetic Resonance Imaging
check_url/fr/59502?article_type=v

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
check_url/fr/59502?article_type=v

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Read Article