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Patient-afledt heterogene xenograft model af kræft i bugspytkirtlen ved hjælp af Zebrafish larver som værter for sammenlignende Lægemiddelvurdering
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1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences,Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery,Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College,Fudan University, 6Pancreatic Cancer Institute,Fudan University
Chapitres
- 00:04Titre
- 01:28Preparing the Equipment for the Microinjection
- 02:21Preparing Embryos for Transplantation
- 02:56Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
- 04:56Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
- 06:29Injecting Mixed Cell Suspension into the Zebrafish
- 07:10Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
- 08:11Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
- 09:12Conclusion
Denne protokol beskriver optimerings procedurer i en virus baseret Dual fluorescens-mærket tumor xenograft model ved hjælp af larve Zebra som værter. Denne heterogene xenograft model efterligner vævet sammensætning af bugspytkirtelkræft mikromiljø in vivo og fungerer som et mere præcist redskab til vurdering af narkotika respons i personlige zPDX (zebrafish patient-afledte xenograft) modeller.
