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Patient-derived heterogena xenograft modell av pankreascancer med Zebrafish larver som värdar för jämförande drog bedömning
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1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences,Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery,Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College,Fudan University, 6Pancreatic Cancer Institute,Fudan University
Chapitres
- 00:04Titre
- 01:28Preparing the Equipment for the Microinjection
- 02:21Preparing Embryos for Transplantation
- 02:56Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
- 04:56Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
- 06:29Injecting Mixed Cell Suspension into the Zebrafish
- 07:10Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
- 08:11Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
- 09:12Conclusion
Detta protokoll beskriver optimerings procedurer i en virusbaserad Dual Fluorescence-märkt tumör xenograft modell med larv zebrafiskar som värdar. Denna heterogena xenograft modell härmar vävnads sammansättningen av pankreascancer mikromiljö in vivo och fungerar som ett mer exakt verktyg för att bedöma läkemedelssvar i personlig zPDX (Zebrafish patient-derived xenograft) modeller.
