Journal
/
Immunologie et infection
/
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

DOI:
10.3791/59531-v

•

10:13 min

•

May 06, 2019

•

, ,
1Department of Microbiology and Immunology,Western University, 2Department of Medicine, Division of Clinical Immunology and Allergy,Western University, 3Department of Surgery, Division of General Surgery,Western University, 4Lawson Health Research Institute

Chapitres

  • 00:04Titre
  • 01:14T Antigen (Ag)-Expressing Tumor Cell Inoculation
  • 02:29Target Splenocyte Preparation
  • 04:10Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
  • 05:44CFSE-Labeling Evaluation
  • 06:50CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
  • 07:31Data Acquisition
  • 08:21Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
  • 09:36Conclusion

Summary

Traduction automatique

Traduction automatique

We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy testing.

Tags

Keywords: In Vivo Cytotoxicity AssayTumor-specific CD8+ T Cell ResponsesImmunodominanceTumor AntigensCytotoxic T Cell ResponsesNK CellsInnate-like T LymphocytesAdherent Tumor Cell LineTarget Splenocyte PreparationC57BL/6 Mice

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59531?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/fr/59531?article_type=v

Competitive Homing Assays to Study Gut-tropic T Cell Migration
check_url/fr/59531?article_type=v

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
check_url/fr/59531?article_type=v

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
check_url/fr/59531?article_type=v

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
check_url/fr/59531?article_type=v

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
check_url/fr/59531?article_type=v

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
check_url/fr/59531?article_type=v

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
check_url/fr/59531?article_type=v

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay
check_url/fr/59531?article_type=v

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Read Article