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从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
DOI:

11:59 min

May 15, 2019

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Chapitres

  • 00:04Titre
  • 01:01Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk
  • 02:04Isolation of LDL Particles from Plasma
  • 03:16Incubation of S. aureus with LDLs for Membrane Lipid Analysis
  • 05:18Extraction of S. aureus Membrane Lipids
  • 06:54Analysis of S. aureus Lipid Profiles using High Resolution/Accurate Mass Spectrometry
  • 08:26Database Searching to Identify Endogenous S. aureus and Exogenous LDL-derived Lipids
  • 09:54Results: Fatty Acid Composition of S. aureus Phospholipids Cultured with Chicken LDLs
  • 11:29Conclusion

Summary

Traduction automatique

该方法为研究将来自复杂宿主来源的外源脂肪酸结合到细菌膜中提供了一个框架,特别是金黄色葡萄球菌。为此,介绍了从鸡蛋蛋黄中浓缩脂蛋白颗粒的方案,以及利用质谱法对细菌磷脂进行随后脂肪酸分析的介绍。

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