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Genotyping of Sea Anemone during Early Development

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Biologie du développement

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Journal JoVE Biologie du développement

Genotyping of Sea Anemone during Early Development

Genotyping of Sea Anemone during Early Development

DOI:

10.3791/59541-v

•

07:04 min

•

May 13, 2019

•

Miguel A.P. Silva, Nagayasu Nakanishi

¹Department of Biological Sciences,University of Arkansas

Chapitres

00:04Titre
00:36Induction of Spawning, In Vitro Fertilization, and De-jellying
01:48Surgical Removal of an Aboral Tissue from a Gastrula Embryo
03:38Genomic DNA Extraction and Genotyping PCR
04:54Results: Genotyping Results of Sea Anemone Embryos
06:07Conclusion

Summary

Traduction automatique

The goal of this protocol is to genotype the sea anemone Nematostella vectensis during gastrulation without sacrificing the embryo.

Tags

Keywords: Genotyping Sea Anemone Early Development Mutant Embryogenesis Phenotype Nematostella Vectensis Spawning Fertilization Dejelly DNA Extraction Agarose Gel Microsurgery

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