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Immunologie et infection

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果蝇、果蝇细胞免疫应答的评估

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Immunologie et infection

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Journal JoVE Immunologie et infection

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

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果蝇、果蝇细胞免疫应答的评估

DOI:

10.3791/59543-v

•

05:50 min

•

April 10, 2019

•

Ashley E. Nazario-Toole², Louisa P. Wu³

¹Department of Biology,University of Maryland, ²Department of Cell Biology and Molecular Genetics,University of Maryland, ³Institute for Bioscience and Biotechnology Research,University of Maryland

Chapitres

00:04Titre
00:30Fly Injection
02:32Fly Imaging and Fluorescence Quantification and Normalization
03:59Results: Representative In Vivo Phagocytosis Assay Analyses
05:18Conclusion

Summary

Traduction automatique

该方案描述了一种在成虫体内吞噬试验的方法, 以量化吞噬细胞的识别和微生物感染的清除。

Tags

Keywords: Phagocytosis Drosophila Melanogaster In Vivo Assay Immune Response Cellular Immunity Genetic Screen Genome-wide Association Study Pathogen Recognition Pathogen Clearance Trypan Blue Fluorescence Microscopy Imaging Analysis

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