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Immunologie et infection
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Vurdering af cellulære immunrespons i frugtflue Drosophila melanogaster, ved hjælp af en i Vivo fagocytose Assay
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Immunologie et infection
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Journal JoVE Immunologie et infection
Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Vurdering af cellulære immunrespons i frugtflue Drosophila melanogaster, ved hjælp af en i Vivo fagocytose Assay

DOI:
10.3791/59543-v

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05:50 min

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April 10, 2019

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,
1Department of Biology,University of Maryland, 2Department of Cell Biology and Molecular Genetics,University of Maryland, 3Institute for Bioscience and Biotechnology Research,University of Maryland

Chapitres

  • 00:04Titre
  • 00:30Fly Injection
  • 02:32Fly Imaging and Fluorescence Quantification and Normalization
  • 03:59Results: Representative In Vivo Phagocytosis Assay Analyses
  • 05:18Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol beskriver en in vivo fagocytose assay i voksen Drosophila melanogaster at kvantificere phagocyt anerkendelse og clearance af mikrobielle infektioner.

Tags

Keywords: PhagocytosisDrosophila MelanogasterIn Vivo AssayImmune ResponseCellular ImmunityGenetic ScreenGenome-wide Association StudyPathogen RecognitionPathogen ClearanceTrypan BlueFluorescence MicroscopyImaging Analysis

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