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Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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Chapitres
- 00:04Titre
- 00:41Whole Blood Peripheral Blood Mononuclear Cell (PBMC) Preparation
- 01:21CD4+ T Cell Preparation
- 03:16CD4+ T Cell Labeling
- 04:11Flow Cytometric Analysis
- 05:31Results: Representative CFSE-labeled, Activated CD4+ T Cell Proliferation
- 06:21Conclusion
Presented here is a protocol for measuring proliferating CD4+ T cells in response to antigenic proteins or peptides using dye dilution. This assay is particularly sensitive to rare antigen-specific T cells and can be modified to facilitate cloning of antigen-specific cells.
