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Recherche en cancérologie
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Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Journal JoVE
Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

DOI:
10.3791/59551-v

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11:39 min

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June 09, 2019

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, , , , , ,
1Shonai Regional Industry Promotion Center, 2Tsuruoka Metabolomics Laboratory,National Cancer Center, 3Human Metabolome Technologies, Inc, 4Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center,National Cancer Center

Chapitres

  • 00:04Titre
  • 01:18Cell Culture on Day 1
  • 04:37Preparation of Reagents and Pre-washing Centrifugal Filter Units
  • 06:17Cell Culture on Day 2
  • 07:24Extraction of Metabolites from Cultured Cells and Ultrafiltration of Cell Extracts
  • 08:55Results: Diamide Treatment
  • 10:42Conclusion

Summary

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The purpose of this article is to describe a protocol for extraction of aqueous metabolites from cultured adherent cells for metabolomic analysis, particularly, capillary electrophoresis-mass spectrometry.

Tags

Keyword Extraction:MetabolomicsCapillary Electrophoresis-mass SpectrometryCEMSAqueous MetabolitesCultured Adherent CellsCancer CellsMetabolite ExtractionBiomarkersChemotherapy ResponseCell CultureTrypsin-EDTACell SuspensionCentrifugation

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