Journal
/
Immunologie et infection
/
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
Journal JoVE
Immunologie et infection
Author Produced
This content is Free Access.
Journal JoVE Immunologie et infection
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia

DOI:
10.3791/59566-v

•

00:13 min

•

September 21, 2019

•

, , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Chapitres

  • 00:01Titre
  • 02:06Intratracheal Instillation
  • 04:46Excision of Infected Lungs
  • 07:16Pathogen Recovery and Analysis
  • 10:03Résultats
  • 12:06Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present methods to improve secondary bacterial pneumonia studies by providing a non-invasive route of instillation into the lower respiratory tract followed by pathogen recovery and transcript analysis. These procedures are reproducible and can be performed without specialized equipment such as cannulas, guide wires, or fiber optic cables.

Tags

Secondary Bacterial PneumoniaMurine ModelPathogen DeliveryPathogen RecoveryTracheal InoculationBacterial CFU EnumerationRNA PurificationVirulence Gene AnalysisMinimally Invasive Technique

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59566?article_type=v

Development of a Negative Selectable Marker for Entamoeba histolytica
check_url/fr/59566?article_type=v

Invasion of Human Cells by a Bacterial Pathogen
check_url/fr/59566?article_type=v

Introducing Shear Stress in the Study of Bacterial Adhesion
check_url/fr/59566?article_type=v

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
check_url/fr/59566?article_type=v

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions
check_url/fr/59566?article_type=v

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
check_url/fr/59566?article_type=v

Measurement of Tactile Allodynia in a Murine Model of Bacterial Prostatitis
check_url/fr/59566?article_type=v

Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
check_url/fr/59566?article_type=v

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
check_url/fr/59566?article_type=v

A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice

Read Article