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Fuldt humant tumor-baserede matrix i tredimensionale Spheroid invasion assay
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Chapitres
- 00:04Titre
- 01:10Multicellular Tumor Spheroid Generation and 3D Spheroid Invasion Assay
- 02:53Image Segmentation With Ilastik
- 04:39Area Analysis with Fiji ImageJ
- 06:23Results: Representative Cell Movement in a 3D Invasion Model
- 07:36Conclusion
Tumor mikromiljø er en væsentlig del af kræft vækst og invasion. For at efterligne karcinomer er der behov for en biologisk relevant menneskelig matrix. Denne protokol introducerer en forbedring for in vitro tredimensionel sfæroide invasion assay ved at anvende en human leiomyoma-baseret matrix. Protokollen introducerer også en computer-baseret celle invasion analyse.
