Journal
/
Médecine
/
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

DOI:
10.3791/59608-v

•

09:34 min

•

September 26, 2019

•

, , ,
1Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS),Saarland University, 2Institute for Clinical and Experimental Surgery,Saarland University

Chapitres

  • 00:04Titre
  • 01:05Primary Cell Culture and Small Interfering RNA (siRNA) Transfection
  • 02:56In Vitro Wound Healing (Scratch Migration) Assay
  • 04:13In Vivo Skin Wound Healing Assay
  • 07:11Results: Representative In Vitro and In Vivo Wound Healing
  • 08:43Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present a protocol for an in vitro scratch assay using primary fibroblasts and for an in vivo skin wound healing assay in mice. Both assays are straightforward methods to assess in vitro and in vivo wound healing.

Tags

Scratch Migration AssayDorsal Skinfold ChamberWound HealingIn VitroIn VivoCell MigrationSkin WoundPharmacologically Active CompoundsFibroblastsGenotypeLipid-based Transfection ReagentSiRNACell CultureConfluencyScratch Wound

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Murine Model of Wound Healing

Murine Endoscopy for In Vivo Multimodal Imaging of Carcinogenesis and Assessment of Intestinal Wound Healing and Inflammation

Ischemic Tissue Injury in the Dorsal Skinfold Chamber of the Mouse: A Skin Flap Model to Investigate Acute Persistent Ischemia

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Read Article