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En muse model af vascularized Heterotopic milt transplantation for at studere milt cellebiologi og transplantations immunitet
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Médecine
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Journal JoVE Médecine
A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

En muse model af vascularized Heterotopic milt transplantation for at studere milt cellebiologi og transplantations immunitet

DOI:
10.3791/59616-v

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08:04 min

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June 11, 2019

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, , , , ,
1Comprehensive Transplant Center,Northwestern University, 2Department of surgery, Feinberg School of Medicine,Northwestern University, 3Weinberg art and science college,Northwestern University

Chapitres

  • 00:04Titre
  • 00:39Donor Spleen Harvest
  • 03:06Recipient Splenectomy and Spleen Graft Implantation
  • 06:28Results: Representative Syngeneic Spleen Transplantation Chimera Generation
  • 07:28Conclusion

Summary

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Denne protokol beskriver de kirurgiske trin af en musemodel af vaskulariseret heterotop milt transplantation, en teknisk udfordrende model, der kan tjene som et kraftfuldt værktøj til at studere skæbne og levetiden af milt celler, mekanismerne i distinkte milt cellepopulationer i sygdomsprogression og transplantations immunitet.

Tags

Mouse ModelVascularized Heterotopic Spleen TransplantationSpleen Cell BiologyTransplant ImmunityCongenic MiceSpleen CellsInflammatory DiseaseSurgical ProcedureDonor MouseAbdominal SurgerySpleen GraftHeparinized SalineCeliac ArteryPortal VeinSplenic Vein

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