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Undersøgelse af organelle dynamik i B-celler under dannelse af immun synapse
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
DOI:

15:39 min

June 01, 2019

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Chapitres

  • 00:00Titre
  • 01:16B Cell Activation with Antigen-coated Beads
  • 02:48B Cell Activation on Antigen-coated Coverslips
  • 03:51Immunofluoresence and Image Analysis
  • 04:30Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads
  • 07:42Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips
  • 09:29Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells
  • 11:23Résultats
  • 15:07Conclusion

Summary

Traduction automatique

Heri beskriver vi to tilgange til at karakterisere celle polariserings hændelser i B-lymfocytter under dannelsen af en IS. Den første, omfatter kvantificering af organelle rekruttering og cytoskelet rearrangementerne på den synaptiske membran. Den anden er en biokemisk tilgang, at karakterisere ændringer i sammensætningen af centrosome, som gennemgår polarisering til immun synapse.

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