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Immunologie et infection
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Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice
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Immunologie et infection
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Journal JoVE Immunologie et infection
Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

DOI:
10.3791/59657-v

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07:39 min

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July 19, 2019

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, , , , , , ,
1The First Affiliated Hospital,Jinan University, 2Department of Biochemistry and Molecular Biology, School of Basic Medical Science,Jinan University, 3The Institute of Clinical Medicine,Jinan University, 4Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

Chapitres

  • 00:04Titre
  • 01:06Embryonic Thymus Isolation
  • 02:54Isolated Embryonic Thymus Culture and Renal Subcapsular Space Establishment
  • 04:36Embryonic Murine Thymus Transplant
  • 05:59Results: Thymus-Transplant Nude Mouse Peripheral Blood T Cell Identification and Evaluation
  • 06:59Conclusion

Summary

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Traduction automatique

We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation.

Tags

Renal Subcapsular TransplantationMurine Embryonic ThymusNude MiceThymus TransplantationThymus Epithelial Cell FunctionT-cell Maturation18.5 Thymus Transplantation2′-deoxyguanosine TreatmentThymic Transplantation

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