Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Nephrology,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 2Shenzhen Xenotransplantation Medical Engineering Research and Development Center,Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine, 3Department of Medical Laboratory,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 4Department of Endocrinology,Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University, 5Xenotransplantation Program, Department of Surgery,University of Alabama at Birmingham, 6People’s Hospital of Longhua
Chapitres
- 00:04Titre
- 00:44Aorta Isolation
- 02:21Porcine Aortic Endothelial Cell (pAEC) Isolation
- 05:16Results: Representative Highly-Purified pAEC Identification
- 05:53Conclusion
Se describe un método enzimático eficaz para el aislamiento de las células endoteliales aórticas porcinas primarias (pAEC) de los cerdos en miniatura. Los pAEC primarios aislados se pueden utilizar para investigar la respuesta inmunitaria y de la coagulación en el xenotrasplante.
