/
/
Standardisert og skalerbar analyse for å studere Perfusert 3D angiogenic spirende av iPSC-avledet endothelial Cells in vitro
This content is Free Access.
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine,Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics,Leiden University, 3Ncardia, 4Department of Anatomy and Embryology,Leiden University Medical Center, 5Mimetas
Chapitres
- 00:04Titre
- 01:12Preparation of Device, Gel, and Coating
- 02:42Cell Seeding/Microvessel Culture
- 04:37Study Angiogenic Sprouting
- 05:49Study Anastomosis and Sprout Stabilization
- 06:50Fixation, Staining, and Imaging
- 08:55Results: Cell Seeding and 3D Sprouting of iPSC-EC
- 10:13Conclusion
Denne metoden beskriver kulturen i iPSC-avledet endothelial celler som 40 perfusert 3D microårene i en standardisert mikrovæskebasert plattform. Denne plattformen muliggjør studiet av gradient-drevet angiogenic spirende i 3D, inkludert anastomose og stabilisering av angiogenic spirer i en skalerbar og høy gjennomstrømming måte.
