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Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

DOI:
10.3791/59716-v

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06:36 min

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June 05, 2019

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, , , ,
1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Manchester Academic Health Science Centre,University of Manchester, 2Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre,University of Manchester, 3Lydia Becker Institute of Immunology and Inflammation,University of Manchester

Chapitres

  • 00:04Titre
  • 00:35Day 0: Egg Production and Collection
  • 01:11Day 1 – 2: Atorvastatin Treatment and ICH- and ICH+ Populations Separation
  • 02:22Day 3 – 5: Cell Death, Leukocyte Analysis and Motility Assay
  • 04:24Results: Intracerebral Hemorrhage (ICH) in Zebrafish Larvae
  • 05:39Conclusion

Summary

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Here we present a protocol to quantify brain injury, locomotor deficits and neuroinflammation following bleeding in the brain in zebrafish larvae, in the context of human intracerebral hemorrhage (ICH).

Tags

Keywords: Zebrafish LarvaeHemorrhagic StrokePre-clinical ApproachCellular ResponseFluorescent MicroscopyAtorvastatin TreatmentHemorrhaged FishFluorescent ProteinLight Sheet MicroscopyAnesthetizationLow Melt AgaroseMounting Capillary

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