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Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

DOI:
10.3791/59744-v

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06:51 min

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December 13, 2019

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, ,
1Molecular Neuro-oncology Laboratory,Brown University, Rhode Island Hospital, 2University of Minnesota, College of Veterinary Medicine, 3Department of Neurosurgery,Brown University

Chapitres

  • 00:04Titre
  • 00:36Compartmented Culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs) and Human Glioma Cells (hGCs)
  • 03:17Culture in the Compartmented Chamber
  • 04:14Results: hGCs in DRG Axons
  • 05:52Conclusion

Summary

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Traduction automatique

Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

Tags

Real-time MonitoringHuman Glioma Cell MigrationDorsal Root GanglionAxon-oligodendrocyte Co-culturesEx Vivo Co-culture SystemCellular And Molecular MechanismsIn Vitro Drug Efficacy TestingCompartmented Culture DishesCollagen CoatingAmmonium HydroxideSilicone GreaseBlunt-tip NeedleSupplemented Neuronal Basal Medium

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