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Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
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Journal JoVE Neurosciences
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

DOI:
10.3791/59797-v

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07:35 min

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August 06, 2019

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,
1Laboratory of RNA Biology,Institute of Molecular Genetics of the Czech Academy of Sciences, 2Department of Genetics and Microbiology, Faculty of Science,Charles University

Chapitres

  • 00:04Titre
  • 00:34Injection
  • 02:54Cell Fixation and Staining
  • 04:07Microscopy
  • 05:09Results: Analysis of Spliceosomal snRNA Localization in Human Hela Cells
  • 07:00Conclusion

Summary

Traduction automatique

Traduction automatique

Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

Tags

Spliceosomal SnRNAMicroinjectionRNA LocalizationCajal BodiesHeLa CellsFluorescently Labeled RNARNA SequenceNuclear StructuresAdherent CellsMicroloaderMicroinjectorMicroscopeObjectiveCell ContactLower LimitCell Injection

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