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Purification of Platelets from Mouse Blood
Journal JoVE
Biologie
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Journal JoVE Biologie
Purification of Platelets from Mouse Blood
DOI:

05:41 min

May 07, 2019

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Chapitres

  • 00:04Titre
  • 00:37Platelet Purification
  • 01:31Platelet Activation
  • 02:51Flow Cytometric Platelet Analysis
  • 03:30Results: Representative Platelet Purification Evaluation
  • 05:03Conclusion

Summary

Traduction automatique

Here, mouse blood was collected in the presence of an anti-coagulant. The platelets were purified by iohexol gradient medium using low speed centrifugation. The platelets were activated with thrombin to investigate if they were viable. The quality of the purified platelets was analyzed by flow cytometry and microscopy.

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