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Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

DOI:
10.3791/59807-v

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08:19 min

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July 20, 2019

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, , , ,
1Department of Otorhinolaryngology, University Hospital,University of Duisburg-Essen

Chapitres

  • 00:04Titre
  • 00:58Allogenic Tumor Mouse Model and Neutrophil Adoptive Transfer
  • 02:32Tumor Growth Evaluation
  • 03:54Tumor-Associated Neutrophil (TAN) Isolation
  • 06:04In Vitro FK866-TAN Inhibition
  • 06:44Results: Representative Analyses of FK866 Treatment on Neutrophil Angiogenic Properties and Tumor Growth
  • 07:32Conclusion

Summary

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Here, we show therapeutic potential of anti-angiogenic tumor-associated neutrophils after their transfer into tumor-bearing mice. This protocol can be used to manipulate neutrophil activity ex vivo and to subsequently evaluate their functionality in vivo in developing tumors. It is an appropriate model for studying potential neutrophil-based immunotherapies.

Tags

Keywords: Tumor-associated NeutrophilsAngiogenic PotentialNAMPT Inhibitor FK866B16F10 MelanomaAdoptive TransferTumor-bearing MiceTumor GrowthHistological AnalysisCryosectioning

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