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In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

DOI:
10.3791/59831-v

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07:10 min

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June 28, 2019

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, , , , , , ,
1Physics of Synthetic Systems – E14, Physics-Department and ZNN,Technische Universität München, 2Departmant of Chemistry, Center for Integrated Protein Science (CIPSM),Technische Universität München, 3Nanosystems Initiative Munich

Chapitres

  • 00:04Titre
  • 00:29Vesicle Production Using the Glass Beads Method
  • 02:24Transcription-translation (TX-TL) Reaction
  • 05:07Results: Analysis of Synthesized Peptide Membrane Precursors
  • 06:41Conclusion

Summary

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Presented here are protocols for the creation of peptide-based small unilamellar vesicles capable of growth. To facilitate in vesiculo production of the membrane peptide, these vesicles are equipped with a transcription-translation system and the peptide-encoding plasmid.

Tags

Peptide Membrane PrecursorsAutonomous Vesicle GrowthFilm Re-hydrationReaction CompartmentsELP SolutionChloroform MethanolGlass BeadsRotary EvaporatorVesicle FormationPlasmid DNA PurificationPhase SeparationEthanol Precipitation

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