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Comparative Lesions Analysis Through a Targeted Sequencing Approach
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Sciences du comportement
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Journal JoVE Sciences du comportement
Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

DOI:
10.3791/59844-v

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08:16 min

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November 05, 2019

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, , , , , , , ,
1ARC-Net Research Centre,University and Hospital Trust of Verona, 2Department of Diagnostics and Public Health, Section of Pathology,University and Hospital Trust of Verona, 3Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit,University of Padua

Chapitres

  • 00:04Titre
  • 00:48Library Pooling and Sequencing Run
  • 02:28Sample Library Dilution and Loading
  • 03:00Next Generation Sequencing
  • 03:43Mutation and Copy-Number Variation (CNV) Analysis
  • 05:00Somatic Mutation Indexing
  • 05:36Results: Representative Comparative Lesion Analyses
  • 07:16Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a method to identify clonal and subclonal alterations among different specimens from a given patient. Although the experiments described here focus on a specific tumor type, the approach is broadly applicable to other solid tumors.

Tags

Targeted SequencingGenetic RelatednessMolecular AlterationsDisease ProgressionIntratumor HeterogeneityAnti-tumor StrategiesSolid Tumor TypesSequencing RunIon Proton SystemIon Gene Studio S5 SystemIon AmpliSeq 2.0 Library KitIon ChefCoverage Analysis PluginGRCH37HG19 GenomeBarcode SetClonal AmplificationNext Generation Sequencing

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