Journal
/
Biochimie
/
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

DOI:
10.3791/59886-v

•

13:22 min

•

October 23, 2019

•

,
1Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute,Arizona State University, 2Department of Pediatrics, and Biochemistry/Molecular Biology, College of Medicine,Pennsylvania State University

Chapitres

  • 00:05Titre
  • 01:01DNA Preparation
  • 04:00Aminosilane Slide Coating and Array Sample Preparation
  • 05:29Microarray Printing and Measurement of DNA levels on NAPPA Slides
  • 06:24Expression of NAPPA Arrays
  • 07:29Detection of Proteins on NAPPA Arrays
  • 08:23Tyrosine Kinase Inhibitor Screening on NAPPA Arrays
  • 10:41Results: Protein Expression
  • 12:25Conclusion

Summary

Traduction automatique

Traduction automatique

A detailed protocol for the generation of self-assembled human protein microarrays for the screening of kinase inhibitors is presented.

Tags

Keywords: NAPPAProtein MicroarrayKinase Inhibitor ScreeningHuman ProteinProtein ActivityProtein FunctionHigh-throughputBiomarker DiscoveryProtein-protein InteractionsSubstrate IdentificationDrug ScreeningQuality ControlBacterial GrowthHigh-throughput Mini-prepTerrific Broth MediumAmpicillin96-pin DeviceDeep Well PlateShakerBacterial LysisAnion Exchange Resin Plates

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/59886?article_type=v

Assaying Protein Kinase Activity with Radiolabeled ATP
check_url/fr/59886?article_type=v

Analysis of Histone Antibody Specificity with Peptide Microarrays
check_url/fr/59886?article_type=v

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
check_url/fr/59886?article_type=v

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
check_url/fr/59886?article_type=v

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
check_url/fr/59886?article_type=v

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation
check_url/fr/59886?article_type=v

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
check_url/fr/59886?article_type=v

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
check_url/fr/59886?article_type=v

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
check_url/fr/59886?article_type=v

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Read Article