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Immunology and Infection

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JoVE Journal Immunology and Infection
Inducerad differentiering av M-Cellliknande celler i humana stamcells-härledda ileal Enteroid Monolayers
 
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Inducerad differentiering av M-Cellliknande celler i humana stamcells-härledda ileal Enteroid Monolayers

Article DOI: 10.3791/59894-v • 11:34 min • July 26th, 2019
July 26th, 2019 •
1, 2, 2, 3
1Program in Immunology, Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology, Baylor College of Medicine, 3Department of Molecular Biology and Microbiology, Tufts University

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Detta protokoll beskriver hur man inducerar differentieringen av M-celler i mänskliga stamcells-härledda ileal enskiktslager och metoder för att bedöma deras utveckling.

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Immunologi och infektion ileal enteroider stamceller polariserande epitelial monolayers Transwells M celler immunofluorescens bägaren celler RANKL GP2 SpiB lgA Lgr5
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