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用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络

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Bio-ingénierie

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Journal JoVE Bio-ingénierie

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

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用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络

DOI:

10.3791/59906-v

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08:53 min

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July 15, 2019

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Viviana Zlochiver*¹, Stacie L. Kroboth*¹, Christopher R. Beal, Jonathan A. Cook, Rosy Joshi-Mukherjee^2,3

¹Aurora Research Institute,Advocate Aurora Health Care, ²Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, ³Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Chapitres

00:04Titre
00:56Pre-plating of the Cryopreserved hiPSC-CM for Maturation
01:35Multiwell MEA Plate Sterilization and Coating
02:57hiPSC-CM Dissociation and Plating on Multiwell MEA Plate
04:09hiPSC-CM Electroporation and Signal Acquisition
05:06Data Segmentation and Analysis
06:40Results: Action Potential Recordings of hiPSC-CMs
08:21Conclusion

Summary

Traduction automatique

本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。

Tags

Keywords: Human IPSC-derived Cardiomyocytes Multiwell Micro-electrode Arrays Action Potential Recordings Cell Plating Electrophysiology MATLAB Workflow Signal-to-noise Ratio Fibronectin Coating

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