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Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage
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Chapitres
- 00:00Introduction
- 01:36Ultraviolet C (UV-C) Radiation Exposure to Mouse Primary Ocular Cells
- 03:43Preparation of the Living-Cell Staining Media
- 04:58Live Cell Staining of UV-C Exposed Mouse Primary Ocular Cells
- 05:51Viewing of DCFDA (ROS), Hoechst and PI Stained Cells
- 07:40Résultats
- 11:57Conclusion
This protocol demonstrates the simultaneous detection of reactive oxygen species (ROS), live cells, and dead cells in live primary cultures from mouse ocular surface cells. 2',7'-Dichlorofluoresceindiacetate, propidium iodide, and Hoechst staining are used to assess the ROS, dead cells, and live cells, respectively, followed by imaging and analysis.
