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Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry
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Journal JoVE Biologie
Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

DOI:
10.3791/59930-v

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07:17 min

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October 30, 2019

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, ,
1Department of Neuroscience, Cell Biology, and Physiology,Wright State University, 2Department of Surgery, Boonshoft School of Medicine,Wright State University

Chapitres

  • 00:05Titre
  • 00:30Vascular Smooth Muscle Cell Labelling with EdU and Fixing with PFA
  • 01:53Detection of EdU Using Fluorescence
  • 02:50Detection of EdU Using Luminescence
  • 04:40Results: Proliferation of Vascular Smooth Muscle Cells Measurements
  • 06:06Conclusion

Summary

Traduction automatique

Traduction automatique

Proliferation is a critical part of cellular function, and a common readout used to assess potential toxicity of new drugs. Measuring proliferation is, therefore, a frequently used assay in cell biology. Here we present a simple, versatile method of measuring proliferation that can be used in adherent and non-adherent cells.

Tags

Keywords: Vascular Smooth Muscle CellsCell ProliferationClick ChemistryEdUFluorescenceLuminescenceCell AssayPDGFParaformaldehydeTX-100THPTACopper SulfateFluorescein Picolyl-azideSodium AscorbateDAPIHRP AzideELISA Substrate

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