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Perakende Gıda Numunelerinde Clostridium Perfringens Toxinotypes Zenginleştirme ve Algılama
Journal JoVE
Environnement
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Journal JoVE Environnement
Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
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Perakende Gıda Numunelerinde Clostridium Perfringens Toxinotypes Zenginleştirme ve Algılama

DOI:
10.3791/59931-v

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08:00 min

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October 18, 2019

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, ,
1Brain and Mind Institute,Weill Cornell Medical College

Chapitres

  • 00:04Titre
  • 00:42Media Preparation and Sample Collection
  • 02:48TSC Sandwich Plating
  • 04:05Sub-culturing of Sulfite-Reducing Colonies
  • 05:08DNA Extraction and PCR Genotyping
  • 07:29Conclusion

Summary

Traduction automatique

Traduction automatique

Bu protokolün amacı, anaerobik odacıklar kullanılmadan, özellikle b ve D gerinim tipleri üreten epsilon toksini olmak üzere, yerel olarak satın alınan gıdalarda farklı Clostridium perfringens toksinlerini tespit etmektir.

Tags

Clostridium PerfringensToxinotypesEnrichmentDetectionRetail Food SamplesEpsilon ToxinModified Rapid Perfringens Medium (RPM)D-cycloserineTryptose Sulfite Cycloserine (TSC) AgarAnaerobic CultureSpore Selection

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