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Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
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Médecine
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Journal JoVE Médecine
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

DOI:
10.3791/59999-v

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11:07 min

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July 06, 2019

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, , ,
1Department of Anesthesiology and Intensive Care Medicine,University Hospital Bonn

Chapitres

  • 00:04Titre
  • 00:46Acute Lung Injury Induction
  • 02:56Blood Sampling, Bronchoalveolar Lavage, Organ Harvesting
  • 05:58Tissue Preparation for FACS Analysis
  • 08:57Results: Validation of Murine Acute Lung Injury Model Against Control Animals
  • 10:31Conclusion

Summary

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Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

Tags

Keywords: Acute Lung InjuryMouse ModelIntratracheal InstillationLipopolysaccharide (LPS)Animal ResearchLung InflammationReproducible TechniqueMinimally Invasive

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