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Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
 

Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation

Article DOI: 10.3791/59999-v 11:07 min July 6th, 2019
July 6th, 2019

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résumé

Presented here is a step-by-step procedure to induce acute lung injury in mice by direct intratracheal lipopolysaccharide instillation and to perform FACS analysis of blood samples, bronchoalveolar lavage fluid, and lung tissue. Minimal invasiveness, simple handling, good reproducibility, and titration of disease severity are advantages of this approach.

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Keywords: Acute Lung Injury Mouse Model Intratracheal Instillation Lipopolysaccharide (LPS) Animal Research Lung Inflammation Reproducible Technique Minimally Invasive
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