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Recherche en cancérologie
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Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
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Journal JoVE Recherche en cancérologie
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

DOI:
10.3791/60022-v

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07:01 min

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March 01, 2020

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, , , , , , ,
1Institute of Biophysics, Faculty of Medicine,University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine,University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine,University of Coimbra, 6Gynecology A Service,Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine,University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine,University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service,Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School,Laboratory Biomedical Sciences

Chapitres

  • 00:05Introduction
  • 00:35Sphere Formation and Derived Adherent Population Generation
  • 02:28Sphere-forming Capacity Determination
  • 03:02Self-renewal Determination
  • 03:59Sphere Projection Area Determination
  • 04:51Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
  • 06:29Conclusion

Summary

Traduction automatique

Traduction automatique

The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.

Tags

Keywords: Cancer Stem CellsCancer Stem Cell SpheresSphere-forming AssayPolyHEMANon-adherent Suspension CultureEpidermal Growth FactorBasic Fibroblast Growth FactorSphere-forming CapacityAdherent SpheresHemocytometerGynecological CancerBreast Cancer

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