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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
This content is Free Access.
1Centre for Craniofacial and Regenerative Biology,King’s College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research,University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics,King’s College London, 4Department of Biological Applications and Technology,University of Ioannina
Chapitres
- 00:04Titre
- 01:05Early Somite Stage Embryo Dissection (Day 1)
- 03:12Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
- 04:36Neural Crest Single Cell Tracking
- 05:22Quantification of Neural Crest Cell Migratory Capacity
- 06:04Quantification of Neural Crest Cell Area and Circularity
- 07:00Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
- 08:43Conclusion
Dieses Protokoll beschreibt die Zerlegung und Kultur von kranialen neuralen Kammzellen aus Mausmodellen, in erster Linie für die Untersuchung der Zellmigration. Wir beschreiben die verwendeten Live-Bildgebungstechniken und die Analyse von Geschwindigkeits- und Zellformänderungen.
