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1Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research,Albert Einstein College of Medicine, 2Departments of Cell Biology and Stem Cell Institute,Albert Einstein College of Medicine, 3Department of Medicine,Albert Einstein College of Medicine, 4Albert Einstein Cancer Center and Diabetes Research Center,Albert Einstein College of Medicine
Chapitres
- 00:04Titre
- 00:43Bone Marrow Isolation
- 02:22Immunostaining for Detection of Hematopoietic Stem Cells
- 03:28TMRM Staining
- 04:17Flow Cytometry
- 05:38Results: Flow Cytometry Analysis of Mitochondrial Membrane Potential
- 06:33Conclusion
Xenobiotiske effluks pumper er meget aktive i hæmatopoietisk stilk og progenitorceller (hspc'er) og forårsage ekstrudering af tmrm, en mitokondriel membran potentielle fluorescerende farvestof. Her præsenterer vi en protokol til præcist at måle mitokondrie membran potentiale i hspcs af tmrm i nærværelse af verapamil, en effluks pumpe hæmmer.
