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Immunologie et infection
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Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation
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Immunologie et infection
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Journal JoVE Immunologie et infection
Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

Purification of Human S100A12 and Its Ion-induced Oligomers for Immune Cell Stimulation

DOI:
10.3791/60065-v

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12:55 min

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September 29, 2019

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, ,
1Department of Pediatric Rheumatology and Immunology,University Children’s Hospital

Chapitres

  • 00:05Titre
  • 01:02Anion-exchange Chromatography
  • 03:53Calcium-dependent Hydrophobic-interaction Chromatography (HIC)
  • 05:26Removal of Endotoxin and Oligomer Separation
  • 07:20Functional Testing on Monocytes
  • 10:22Results: Oligomers Separation
  • 11:59Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes a purification method for recombinant tag-free calcium binding protein S100A12 and its ion-induced oligomers for human monocyte stimulation assays.

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