Journal
/
Biologie
/
A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency
Journal JoVE
Biologie
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

DOI:
10.3791/60087-v

•

07:43 min

•

October 05, 2019

•

, , , , , , ,
1Interfaculty Institute of Biochemistry,University of Tübingen, 2Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine,University of Tsukuba, 3Department of Pediatrics, Medical Faculty Mannheim,Heidelberg University

Chapitres

  • 00:00Titre
  • 01:59Protocol
  • 05:54Résultats
  • 07:08Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we demonstrate how to set up an inexpensive volt-amperemeter with programmable output frequency that can be used with commercially available chopstick electrodes for transepithelial/endothelial electrical resistance measurements.

Tags

TEER MeasurementTransepithelial Electrical ImpedanceVolt-ammeterProgrammable Output FrequencyCell CultureBlood-cerebrospinal Fluid BarrierIn Vitro ModelChopstick ElectrodeEpithelial Volt-ammeterUSB ChargerMicrocontrollerSquare Wave CurrentMultimetersRJ14 ConnectorLow-budget

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Microfabricated Post-Array-Detectors (mPADs): an Approach to Isolate Mechanical Forces

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo

Immunocytochemical Analysis of Human Pluripotent Stem Cells using a Self-Made Cytospin Apparatus

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module

Read Article